Sleep, a fundamental and complex physiological process, is integral to overall well-being, with its significance extending beyond the realms of rest and rejuvenation. Concurrently, oral health, encompassing the condition of the oral cavity and its structures, plays a pivotal role in an individual’s general health status. While these two domains may appear disparate at first glance, the evidence suggests a multifaceted relationship, whereby disturbances in one may significantly impact the other. Effect of sleep on oral health has been studied and documented, but the converse has not been focused. Research on the impact of oral biome-brain axis is still in the nascent stage. Understanding the intricate relationship between sleep quality and oral health holds significant clinical and public health implications. Unraveling the underlying mechanisms and identifying potential intervention strategies could pave the way for comprehensive approaches to improve both sleep quality and oral health, ultimately enhancing overall well-being. The proposed study will be carried out to assess the effect of night-time brushing on sleep quality of 80 healthy children between 08-18 years of age using under-the-mattress sleep analyzer.