The primary objective of my clinical trial is "Can collagen peptide supplement enhance the effect of non-surgical periodontal therapy and decrease the level of salivary nitric oxide in chronic periodontitis patients?" 

METHODOLOGY Study design:

The present study is a double blind, parallel group, clinico-biochemical, randomised controlled trial with a 2-months period.

 Group A: 23 Chronic periodontitis patients with collagen peptide supplement as an adjunct to scaling and root planing and salivary nitric oxide level.

 Group B: 23 Chronic periodontitis patients with scaling and root planing alone and salivary nitric oxide level.

Study setting:

Subjects with chronic mild to moderate periodontitis will be selected for the study from the out-patient department, Department of Periodontology.

Sample size:

A sample size of minimum 46 patients (Group A= 23 and Group B= 23) is selected for the study.

Clinical procedure: 1^st visit: baseline

 1.     Informed consent from the subject will be obtained. 

2.     At 1^st visit, detailed medical, dental histories as well as periodontal examination will be recorded and patients fulfilling the inclusion criteria will be included in the study.

3.     Unstimulated saliva samples will be taken to evaluate the salivary nitric oxide level.

4.     Thorough scaling and root planing will be done for all patients.

5.     Test group patients will be given hydrolyzed collagen peptide powder sachets of 5g/day for two months that should be taken in water half an hour before meal (lunch).

Nitric oxide level in saliva:

Salivary estimation of NO will be carried out using the Griess colorimetric reaction in the laboratory. 

2^nd visit: 4 weeks

Follow up and evaluation of clinical parameters.

 3^rd visit: 8 weeks

Follow up and evaluation of clinical parameters.

Unstimulated saliva sample collection to re-evaluate salivary nitric oxide level.