1. Good general health without any known allergy to commercial dental products.

2. The teeth are free of decay or restoration.

3. Patients have not previously received any dentine hypersensitivity treatments.

4. Patients have no history of receiving any teeth whitening treatments.

5. The degree of tooth initial sensitivity should be 3 or higher based on VAS.

Participants excluded from the study had the following conditions:

1. use of medications that could interfere with the perception of pain including chronic

use of anti-inflammatory, analgesic, anticonvulsant, sedative, or other psychotropic drugs

and pregnancy or breastfeeding and patients with chronic diseases.

2. Patients who have been using desensitizing toothpaste or mouthwash in the last 3 months.

3. Patients who had underwent periodontal surgery in the last 6 months.

4. Teeth with evidence of pulpitis, Vital or non-vital teeth that have cracks, previous large restorations, deep abrasions requiring Class V fillings, or had any fractured or endodontically treated teeth carious lesions, gingival recession of more than 3 mm or active periodontal disease.

5. Teeth with deep carious lesions in the vicinity of sensitive teeth that their pain can be confused with tooth sensitivity.