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CTRI Number  CTRI/2024/05/066882 [Registered on: 07/05/2024] Trial Registered Prospectively
Last Modified On: 03/05/2024
Post Graduate Thesis  Yes 
Type of Trial  Interventional 
Type of Study   Ayurveda
Surgical/Anesthesia 
Study Design  Randomized, Parallel Group Trial 
Public Title of Study   Study On Skin Graft With And Without Leech And Mamsarohini Twaka Kwatha Prakshalana As Pretreatment In Management Of Ulcer Which Not Healed Since Long Term 
Scientific Title of Study   A Clinical Study On Split Thickness Skin Graft(STSG) With And Without Jalaukavacharana And Mamsarohini Twaka Kwatha Prakshalana As Pre-Treatment In The Management Of Dushta Vrana (Chronic Non Healing Ulcer) 
Trial Acronym  NIL 
Secondary IDs if Any  
Secondary ID  Identifier 
NIL  NIL 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Rutvij Sunilkumar Bhatt 
Designation  PG Scholar 
Affiliation  Government Akhandanand Ayurved College and Hospital 
Address  Department of Shalya Tantra OPD 24 Government Akhandanand Ayurved College And Hospital Bhadra Ahmedabad Gujarat 380001 India

Ahmadabad
GUJARAT
380001
India 
Phone  7043852727  
Fax    
Email  rutvijbhatt27@gmail.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Harshit S Shah 
Designation  Pricipal and Professor 
Affiliation  Government Akhandanand Ayurved College and Hospital 
Address  Department of Shalya Tantra OPD 24 Government Akhandanand Ayurved College And Hospital Bhadra Ahmedabad Gujarat 380001 India

Ahmadabad
GUJARAT
380001
India 
Phone  9824068160  
Fax    
Email  sejharsh@gmail.com  
 
Details of Contact Person
Public Query
 
Name  Dr Harshit S Shah 
Designation  Pricipal and Professor 
Affiliation  Government Akhandanand Ayurved College and Hospital 
Address  Department of Shalya Tantra OPD 24 Government Akhandanand Ayurved College And Hospital Bhadra Ahmedabad Gujarat 380001 India

Ahmadabad
GUJARAT
380001
India 
Phone  9824068160  
Fax    
Email  sejharsh@gmail.com  
 
Source of Monetary or Material Support  
Government Akhandanand Ayurved College and Hospital Ahmedabad Gujarat 380001 India 
 
Primary Sponsor  
Name  Government Akhandanand Ayurved College and Hospital  
Address  Department of Shalya Tantra OPD 24 Government Akhandanand Ayurved College And Hospital Bhadra Ahmedabad Gujarat 380001 India 
Type of Sponsor  Government medical college 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Rutvij S Bhatt  Government Akhandanand Ayurved College and Hospital  Department of Shalya Tantra OPD 24 Government Akhandanand Ayurved College And Hospital Bhadra Ahmedabad Gujarat 380001 India
Ahmadabad
GUJARAT 
7043852727

rutvijbhatt27@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
INSTITUTIONAL ETHICAL COMMITTEE  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition:L00-L99||Diseases of the skin and subcutaneous tissue. Ayurveda Condition: DUSHTAVRANAH,  
 
Intervention / Comparator Agent  
snoIntervention/ComparatorTypeDrug-TypeProcedure NameDetails
1Comparator ArmProcedure-sandhAna-karma, सन्धान-कर्म (Procedure Reference: , Procedure details: Debridement on 1st Day (Under Suitable Anaesthesia). Standard Dressing For 2nd to 6th day. Frequency: once in a day. Route of application: Topical. Procedure of Spilt Thickness Skin Graft on 7th Day.)
2Intervention ArmProcedure-sandhAna-karma, सन्धान-कर्म (Procedure Reference: susrut samhita chikitsasthan 1/45, susruta samhita sutrasthan 16/27-28,susruta samhita sutrasthan 13, Procedure details: Debridement On 1st Day (Under Suitable Anaesthesia). Jalaukavacharana Followed by Prakshalan With Mamsarohinitwaka Kwath For 2nd to 6th Day. Frequency: Once in a day. Route of Administration: topical. Number of leeches depand on size of Dustavrana. Dushtavrana of about to 3×3 cm:1 leech. Dushtavrana of about to 5×5 cm:2 leeches. Dushtavrana of about to 7×7 cm:3 leeches. After Jalaukavcharan, Dusthavran will be washed with sufficient quantity of Mamsarohinitwaka kwath. Procedu)
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  60.00 Year(s)
Gender  Both 
Details  Patient with single ulcer with size less than 7×7 cm will be include in study
HbA1c level ≤ 7%
Patient with blood pressure ≤ 150/90mmHg
Venous ulcer
Diabetic ulcer
Traumatic wound which fail to heal
Infectious ulcer
Burns
Pressure sore 
 
ExclusionCriteria 
Details  Patients with infectious diseases like HBsAg AIDS Tuberculosis
Leprosy
Arterial Ulcers
Patients associated with disease of kidney and liver
Patients with any coagulopathy like Haemophilia
Vulnerable group of people like mentally ill
Immunocompromised patients
Pressure sore on sole 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Not Applicable 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
Effect Of Two Therapies on acceptance and Rejection Of Graft  60 Days 
 
Secondary Outcome  
Outcome  TimePoints 
Effect Of Two Therapies on accepted & Rejected Graft  60 Days 
 
Target Sample Size   Total Sample Size="14"
Sample Size from India="14" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 3/ Phase 4 
Date of First Enrollment (India)   15/05/2024 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="2"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Not Yet Recruiting 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary   RESEARCH QUESTION
 Whether the Jalaukavcharanaa and Mamsarohini twaka Kwatha prakshalana as Pre-treatm ent of Split Thickness Skin Graft(STSG) In The Management Of Dushta Vrana (chronic non healing ulcer) is useful to reduce rejection of graft or not?

HYPOTHESIS
 Null Hypothesis (H0) –There is no significant difference in acceptance of graft with and without Jalaukavacharanaa and Mamsarohini twaka kwath prakshalana as pre-treatment of Split Thickness Skin Graft (STSG) in the surgical management of Dushtavrana (Chronic non healing ulcer). 
Alternative Hypothesis (H1)– There is significant difference in acceptance of graft with and without Jalaukavacharanaa and Mamsarohini twaka kwath prakshalana as pre-treatment of Split Thickness Skin Graft (STSG) in the surgical management of Dushtavrana (Chronic non healing ulcer).

AIM
To Evaluate and compare the effect , with and without Jalaukavacharana and Mamasarohini twaka Kwatha prakshalana as Pre-treatment in the Acceptance or Rejection of Split Thickness Skin Graft (STSG).

OBJECTIVES 
• To Evaluate the Effect of Jalaukavacharana and Mamasarohini twaka Kwatha prakshalana as Pre-treatment of Split Thickness Skin Graft (STSG) in the surgical management of Dushta vrana (Chronic non healing ulcer). 
• To Evaluate the Acceptance or Rejection of graft with Jalaukavcharna and Mamsarohini twaka Kwatha Prakshlana as Pre-treatment of Split Thickness Skin Graft (STSG) in the surgical management Dushta vrana (Chronic non healing ulcer).
 â€¢ To Evaluate the Acceptance or Rejection of graft without Jalaukavacharanaa and 8 Mamasarohini twaka Kwatha Prakshalana as Pre-treatment of Split Thickness Skin Graft (STSG) in the surgical management Dushta vrana (Chronic non healing ulcer). 

PLAN OF THE STUDY 
 REVIEW OF LITERATURE: In this phase of study, various Ayurvedic and modern literatures related to the present research work will be studied thoroughly. Also, the articles related to the present topic published in renowned and reputed journals will be reviewed thoroughly. Under this phase of study, following topics will be reviewed; • DRUG REVIEW A detailed study of Mamsarohini from Ayurvedic texts, research papers etc. will be done and compiled. 

 PROCEDURE REVIEW
 In procedure review, detailed description of Split Thickness Skin Graft (STSG) and Jalaukavacharana will be described on basis of classical texts of Ayurveda and Modern aspect.

 â€¢ DISEASE REVIEW 
 In disease review, detailed description of Dushta vrana(Chronic non healing ulcer) will be described on the basis of classical texts of Ayurveda and modern aspect.

EXPERIMENTAL STUDY:
• Pharmacognostical study: This study will be done for authenticity at the Department of Dravyaguna of Government Akhandanand Ayurved college, Ahmedabad. 

CLINICAL STUDY: It is a clinical study. In this study total 14 patients randomly selected as per inclusion criteria and randomly divided in 2 groups and each group has same number of patients. A special proforma will be prepared incorporating all points of history taking, physical signs and symptoms, clinical examination and investigation.

STUDY DESIGN: 
● Study Type : An Open label Randomized Controlled Clinical Trial 
● No. of Groups : 2 (7 patients in each group) 
● Sample size: : 7 patients in each group 
● Location of study : Study will be conducted at Government Akhandanand Ayurved Hospital, Ahmedabad and Maniben Ayurved Hospital,Ahmedabad. 

ETHICAL CLEARENCE & CTRI REGESTRATION: 

Approval from Institutional Ethics Committee (IEC) for clinical trials will be obtained prior to the initiation of the study. CTRI registration will be done in due course of time. 

- All the patients will be given complete idea about the clinical trial. Patients will be randomly divided into 2 groups by computerized randomization method. 
- The baseline screening will be done by fulfillment of case record form, which will be designed with detailed clinical history, general and physical examination, assessment of involved Dosha, Dushya, Ama, Agni, Srotasa etc., assessment of treatment.
- Prior written informed consent will be taken from the enrolled patients. All the patients will be assessed clinically before and after the treatment. Assessment will be done on the basis of improvement in signs and symptoms of the disease by specific screening patterns. All the subjective symptomatology will be given specific scores.

POSOLOGY OF DRUG:

Drug

Latin Name

Part used

Mamsarohini

Soymida febrifuga A. Juss.

Bark


GROUPING:


 The selected patients will be divided into two equal groups – Group A & Group B 


Group A (Trial Group)

Split Thickness Skin Graft (STSG) with Jalaukavacharana and Mamasarohini twaka Kwatha Prakshalana as pre-treatment in Dushta vrana (Chronic non healing ulcer).

Group B (Control Group)

Split Thickness Skin Graft (STSG) without Jalaukavacharanaa and Mamasarohini twaka Kwatha Prakshalana as pre- treatment in Dushta vrana (Chronic non healing ulcer).


CRITERIA FOR ASSESSMENT

 

Acceptance of STSG will be determined according to following criteria given below:

 

OBJECTIVE CRITERIA: 


No.

Criteria name

Gradation

1.

Adherence of graft

Grade-0:   Fully Adhere 

Grade-1:   Half Adhere

 

 

Grade-2:   Partially Adhere (< Half)

Grade-3:  No Adhere

2.

STRAVA (Discharge)

Grade-0:  No Discharge

Grade-1:  Serous Discharge

Grade-2:  Serosanguinous 

Grade-3:  Pus Discharge  

3.

VARNA (Colour)*

Grade-0: same as normal skin colour

Grade-1:  +1 then normal skin colour Grade-2: +2 then normal skin colour

Grade-3: +3 then normal skin colour 

Grade-4: +4 then normal skin colour

Grade-5: >+4 then normal skin colour


  SUBJECTIVE CRITERIA:

          
          Pain

INVESTIGATIONS :


•       CBC

•       HCV

•       ESR

•       HbA1c

•       HIV 

•       RBS

•       HBsAg

•       BT & CT

•       S.Creatinine (if needed)

•       Billirubin(total,direct,indirect) (if needed)

•       Urine analysis (Physical, Chemical, Microscopic) 

•       LFT (if needed)

•       RFT (if needed)

•       Arterial &Venous Doppler (if needed)

 

STATUS OF ONGOING MEDICINES AND TREATMENT :

If patient is taking any other medicine for any systemic disease, then it will be continued.


WITHDRAWAL CRITERIA:  

•       During the trial period, In case of any complications occurs ,

•       Personal reasons of patients. 

REPORTING ADR:

Adverse Drug Reaction (ADR), if observed will be registered and reported to Peripheral Pharmacovigilance Centre (PPvC), GAAC, Ahmedabad.

STATISTICAL ANALYSIS:  

Mean score, Standard Deviation (S.D.), Standard Error (S.E.), % of relief, common ‘t’ test, Wilcoxon rank sum test , Mann Whitney U test and other suitable statistical tools will be applied to assess the significance of the results.  

STANDARD PROFORMA:  

Standard Proforma will be developed and used in the study to evaluate the comparative efficacy of the trial drug.

PRESENTATION OF DATA:

The data collected, analyzed will be depicted as general observations viz. age, gender, religion etc. The results of individual investigations will be presented by a proper bar diagram, pie diagrams etc.

DISCUSSION AND CONCLUSION:

 

After getting all the data, discussion and conclusion will be done as per the observations and results.

 

DECLARATION:

The study will be completed in the stipulated time limit. The work will be carried out after getting clearance from the institutional ethics committee. The data related to the identification of the patient will be kept confidential. The clinical data obtained will be summarized at the end of the study. 

 
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