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CTRI Number  CTRI/2025/03/082426 [Registered on: 17/03/2025] Trial Registered Prospectively
Last Modified On: 11/03/2025
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Medical Device 
Study Design  Randomized, Parallel Group Trial 
Public Title of Study   A Study Comparing Two Treatments: Direct Glue Injection vs. Targeted Ultrasound-Guided Treatment with Glue and Coils to Stop Bleeding from Swollen Stomach Vein 
Scientific Title of Study   An Open label Randomized controlled trial to assess the efficacy of EUS guided Coiling and Glue injection compared to conventional glue injection (Direct endoscopic injection) in prevention of late rebleed among patients with bleeding gastric varices 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
NIL  NIL 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Sudipta Dhar Chowdhury 
Designation  Professor 
Affiliation  Christian Medical College, Vellore 
Address  Department of Gastroenterology Christian Medical College,Vellore Ranipet Campus Ranipet, Tamil Nadu 632517

Vellore
TAMIL NADU
632517
India 
Phone  9952311988  
Fax    
Email  sudiptadharchowdhury@gmail.com  
 
Details of Contact Person
Scientific Query
 
Name  Sudipta Dhar Chowdhury 
Designation  Professor 
Affiliation  Christian Medical College, Vellore 
Address  Department of Gastroenterology Christian Medical College,Vellore Ranipet Campus Ranipet, Tamil Nadu 632517

Vellore
TAMIL NADU
632517
India 
Phone  9952311988  
Fax    
Email  sudiptadharchowdhury@gmail.com  
 
Details of Contact Person
Public Query
 
Name  Sudipta Dhar Chowdhury 
Designation  Professor 
Affiliation  Christian Medical College, Vellore 
Address  Department of Gastroenterology Christian Medical College,Vellore Ranipet Campus Ranipet, Tamil Nadu 632517

Vellore
TAMIL NADU
632517
India 
Phone  9952311988  
Fax    
Email  sudiptadharchowdhury@gmail.com  
 
Source of Monetary or Material Support  
Society of GI endoscopy of India 
 
Primary Sponsor  
Name  EUS Educational Foundation 
Address  A905, Ashok Gardens, TJ Road, Sewree, Mumbai, 400015 
Type of Sponsor  Other [Not for profit - Non governmental organisation] 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 11  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Pramod Garg  All India Institute of Medical Sciences  Sri Aurobindo Marg, Ansari Nagar, Ansari Nagar East, New Delhi, Delhi 110029 New Delhi DELHI
New Delhi
DELHI 
9810038116

pgarg10@gmail.com 
Dr Sundeep Lakhtakia  Asian Institute of Gastroenterology  Survey No 136, 4/5, Plot No 2/3, Mindspace Rd, P Janardhan Reddy Nagar, Gachibowli, Hyderabad, Telangana 500032 Hyderabad TELANGANA
Hyderabad
TELANGANA 
9848040629

drsundeeplakhtakia@gmail.com 
Dr Sudipta Dhar Chowdhury  Christian Medical College Vellore  Department of Gastroenterology, Christian Medical College, Vellore Ranipet - 632 517, Tamil Nadu, India.
Vellore
TAMIL NADU 
9952311988

sudiptadharchowdhury@gmail.com 
Dr Amol Bapaye  Deenanath Mangeshkar Hospital and Research Center  Department of Gastroenterology, Deenanath Mangeshkar Hospital Road, near Mhatre Bridge, Vakil Nagar, Erandwane, Pune, Maharashtra 411004
Pune
MAHARASHTRA 
9822053654

amolbapaye@gmail.com 
Dr Siddharth Srivastava  G B Pant Institute of Postgraduate Medical Education & Research  Department of Gastroenterology G B Pant Institute of Postgraduate Medical Education & Research (GIPMER) New Delhi- 110002
Central
DELHI 
9718599215

docsiddharth1@gmail.com 
Dr Vinay Dhir  Institute of Digestive and Liver Care  5th floor, S.L. Raheja Hospital, Mahim West, Maharashtra 400016 Mumbai MAHARASHTRA
Mumbai
MAHARASHTRA 
9819920266

vinaydhir@gmail.com 
Dr Rajesh Puri  Medanta Hospital  Department of Gastroenterology, Medanta- The Medicity, CH Baktawar Singh Rd, Medicity, Islampur Colony, Sector 38, Gurugram, Haryana 122001
Gurgaon
HARYANA 
9811638338

purirajesh69@gmail.com 
Dr Jayanta Samanta  Post Graduate Institute of Medical Education & Research  Madhya Marg, Sector 12, Chandigarh, 160012 Chandigarh CHANDIGARH
Chandigarh
CHANDIGARH 
9855319529

dj_samanta@yahoo.co.in 
Dr Praveer Rai  Sanjay Gandhi Post Graduate Institute  Department of Gastroenterology New PMSSY Rd, Raibareli Rd, Lucknow, Uttar Pradesh 226014
Lucknow
UTTAR PRADESH 
9235630450

praveer_rai@yahoo.com 
Dr Pankaj Desai  Surat Institute of Digestive Sciences  Department of Gastroenterology SIDS Hospital & Research Centre, off Ring Road, near Shell Petrol Pump, Sosyo Circle, Lane, Surat, Gujarat 395002
Surat
GUJARAT 
9824100681

drp_desai@hotmail.com 
Dr Sridhar Sundaram  Tata Memorial Hospital  Department of Gastroenterology, Homi Babha Building, Dr. Ernest Borges Road, Parel East, Parel, Mumbai - 400012.
Mumbai
MAHARASHTRA 
9860096846

drsridharsundaram@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 3  
Name of Committee  Approval Status 
Christian Medical College Vellore  Approved 
Institutional Ethics Committee, Maulana Azad Medical College  Approved 
SL Raheja Hospital, Mahim, Mumbai  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: K318||Other specified diseases of stomach and duodenum,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Endoscopic glue injection  Endoscopic injection of cyanoacrylate glue 
Comparator Agent  EUS guided coil and glue injection  Endoscopic ultrasound-guided coil embolization combined with cyanoacrylate glue injection 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  80.00 Year(s)
Gender  Both 
Details  1. Upper GI bleed secondary to bleeding gastric varices
2. Gastric varices amenable to EUS/Endoscopic therapy 
 
ExclusionCriteria 
Details  1. Pregnancy
2. Platelets count less than 50,000 
3. International Normalized Rate (INR) more than  2.
4. Patients with Child C cirrhosis
5. Patients in altered sensorium
6. Hepatorenal syndrome
7. Sepsis
8. Multiorgan failure,
9. Previous endoscopic treatment for GVs,
10. Malignancy
11. Portal and splenic vein thrombosis
12. Oesophageal stricture
 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
To compare the efficacy of DEI of CYA versus EUS guided coiling and glue for control of late rebleeding in patients with bleeding gastric varices  Follow up at 2 weeks, 4 weeks and 12 weeks after therapy 
 
Secondary Outcome  
Outcome  TimePoints 
Early rebleeding
Complications,
Duration of hospital stay
Cost of therapy
 
Early rebleeding - 2 - 5 days of therapy 
 
Target Sample Size   Total Sample Size="110"
Sample Size from India="110" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 3/ Phase 4 
Date of First Enrollment (India)   01/05/2025 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="2"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)   Not Yet Recruiting 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  

Introduction:

Gastric varices (GV) develop as a consequence of portal hypertension. GV bleeding is associated with a higher risk of rebleeding, morbidity and mortality than oesophageal varices. [1] Initial management for gastric variceal bleeding  includes assessment and management of circulatory status, respiratory status and administration of vasoactive drugs and antibiotics. 
In the 1980s Soehendra et al described a technique for endoscopic guided direct injection of gastric varices with bucrylate.[2] Since then direct endoscopic injection (DEI) of cyanoacrylate (CYA) into the gastric varix has been the main stay of therapy.[3] DEI is an effective therapy for managing gastric variceal bleeding. However a number of complications including cast extrusion, sepsis, embolism of glue thrombus, ulceration etc have been reported with DEI of cyanoacrylate. Endoscopic ultrasound (EUS) guided injection of coils and CYA into varices is a recently described modality of treatment of gastric varices. [4] EUS guided therapy has certain advantages of over DEI. With the help of EUS a precise localisation and injection of varices is possible. The use of coil can reduce the volume of CYA required for obliteration of varices. EUS can also confirm the complete obliteration of a target varix.[5]  In a recent multicenter propensity score matched retrospective study, EUS guided coiling and glue injection was found to have a better efficacy compared to DEI in prevention of rebleed. [8] In a recent network meta analysis, compared to DEI the risk of rebleed was lower with EUS guided coil and glue injection. [6] In a randomized controlled trial the risk of rebleed was lower with EUS Glue and coil versus coiling alone. [7] However, there is no randomised study that contrasts the likelihood of a late rebleed after direct endoscopic injection against EUS guide glue and coil. The present study is being done with an aim to compare DEI of CYA to EUS guided coil and CYA in bleeding GV in prevention of late rebleeding in patients with bleeding GV. 

Objective:
1. Primary objective:  To compare the efficacy of DEI of CYA versus EUS guided coiling and glue for control of late rebleeding in patients with bleeding gastric varices 
Rebleeding defined by any of the following criteria:  Death,   Fresh hematemesis or >100 mL of fresh red NG aspirate,   Development of hypovolemic shock, more than 2 g/dL drop in Hb within any 24 hour period (without transfusion)
2. Secondary objectives: early rebleeding (within 2 - 5 days of therapy), complication, duration of hospital stay, cost of therapy
Methods: 
All consecutive patients admitted during the study period with gastric variceal bleed secondary to cirrhosis related portal hypertension at the participating centres will be screened for eligibility and those fulfilling the inclusion criteria will be included in the study
Inclusion criteria: Age > 18 years, a.     Upper GI bleed secondary to bleeding gastric varices, Gastric varices amenable to EUS/Endoscopic therapy (Hashizome’s – F2 and F3)
Exclusion criteria: Pregnancy, Platelets count less than 50,000 /mL and International Normalized Rate (INR) > 2, Patients with Child C cirrhosis, Patients in altered sensorium, Hepatorenal syndrome , Sepsis, Multiorgan failure, Previous endoscopic treatment for GVs, Malignancy, Portal and splenic vein thrombosis, Oesophageal stricture
Setting: tertiary care centre
Study design: The study is designed as a prospective open label randomized controlled trial to assess the efficacy of EUS guided glue and coil injection versus direct endoscopic injection of cyanoacrylate glue in patients with bleeding gastric varices for control of late rebleeding. Consecutive patients will be randomly assigned to either treatment group. 
Study duration: 2 years
Randomisation: Randomization will be done centrally. A computer generated randomization sequence with variable block size will be generated by the Department of Biostatistics at CMC Vellore. The randomization list will be generated for 110 patients (55 in each arm). 
Allocation concealment: Sequentially numbered opaque sealed envelopes containing allocation information will be created by a statistician who is not involved in the study. The study envelopes will be stored centrally in a secure locker and will be opened once a patient is recruited in the study and the participating site obtains consent from the patient. Details of the patient will be noted on the envelop cover
 
Trial procedure:
EUS Glue and Coil: Prior to EUS examination a through endoscopic examination will be done to ascertain the varix location and presence of RCS. Following the endoscopic examination IV ANTIBIOTIC will be administered and a linear EUS scope will be positioned in the distal oesophagus/gastric fundus to assess the varices. Water may be instilled in the gastric fundus to improve the acoustic coupling. The variceal diameter at EUS will be noted (DiameterThe maximum short-axis diameter of GV (mm)). Presence of perforating veins and perigastric veins will also be located and noted. 
For EUS guided coiling; a coil of ~1.2 times the short axis diameter of the gastric varix will be chosen. A 19 G EUS FNAC needle will be used to inject the coil into the varix. This will be followed by injection of cyanoacrylate 0.5 ml – 1 ml through the EUS FNAC needle. Repeat injection may be required if there is incomplete obliteration of the GV on colour doppler. The procedure will be considered complete once there is no flow within the varix
Direct endoscopic glue injection: A through endoscopic examination will be done to ascertain the varix location and presence of RCS. Following the endoscopic examination IV ANTIBIOTIC will be administered. A sclerotherapy needle primed with saline will be used to puncture the GV. 2 ml of distilled water will be then injected to confirm correct needle placement. Following this 1 ml of Cyanoacrylate glue would be injected under endoscopic visualization followed by injection of 2ml of distilled water. The procedure will be considered complete if there is a change in colour of GV or the GV appears firm on gentle probing.
 
Evaluation and Monitoring of participants: 
The procedure details will be recorded (number of coils/amount of CYA) any adverse event related to the procedure will be recorded. The participants will be followed up at 2 weeks/ 4 weeks and 12 weeks after discharge from hospital. A structured questionnaire will be administered to the patients to ascertain likelihood of rebleed during follow up. Routine blood examinations as per standard of care will be done at each follow up visit.
 
Sample size:
In a retrospective propensity score matched study the incidence of rebleed in DEI was 40 % and the proportion of rebleeding in the EUS guided glue and coil injection was 13.8%. [8]
The sample size was estimated under the assumption that the proportion of patients who present with late rebleeding would be 40% in the DEI group and 15% in the EUS guided coil and glue injection group. With an alpha of 0.05 and a power of 0.80 a 2 tailed test and a 10 % drop the number of patients to be included in each arm is 55.
Pre-planned interim analysis: We will do an interim analysis after including half of the sample size is achieved to see any trend towards intervention or futility.  
References:
 
1. Wani ZA, Bhat RA, Bhadoria AS, Maiwall R, Choudhury A. Gastric varices: Classification, endoscopic and ultrasonographic management. J Res Med Sci Off J Isfahan Univ Med Sci. 2015;20:1200–7. 
2. Soehendra N, Nam VC, Grimm H, Kempeneers I. Endoscopic obliteration of large esophagogastric varices with bucrylate. Endoscopy. 1986;18:25–6. 
3. de Franchis R, Bosch J, Garcia-Tsao G, Reiberger T, Ripoll C, Baveno VII Faculty. Baveno VII - Renewing consensus in portal hypertension. J Hepatol. 2022;76:959–74. 
4. Binmoeller KF, Weilert F, Shah JN, Kim J. EUS-guided transesophageal treatment of gastric fundal varices with combined coiling and cyanoacrylate glue injection (with videos). Gastrointest Endosc. 2011;74:1019–25. 
5. Bhatia V. Endoscopic Ultrasound (EUS) for Esophageal and Gastric Varices: How Can it Improve the Outcomes and Reduce Complications of Glue Injection. J Clin Exp Hepatol. 2012;2:70–4. 
6. Samanta J, Nabi Z, Facciorusso A, Dhar J, Akbar W, Das A, et al. EUS-guided coil and glue injection versus endoscopic glue injection for gastric varices: International multicentre propensity-matched analysis. Liver Int Off J Int Assoc Study Liver. 2023;43:1783–92. 
9. Giri S, Jearth V, Seth V, Darak H, Sundaram S. Comparison of efficacy and safety of endoscopic and radiological interventions for gastric varices: A systematic review and network meta-analysis. Clin Exp Hepatol. 2023;9:57–70. 
7. Robles-Medranda C, Oleas R, Valero M, Puga-Tejada M, Baquerizo-Burgos J, Ospina J, et al. Endoscopic ultrasonography-guided deployment of embolization coils and cyanoacrylate injection in gastric varices versus coiling alone: a randomized trial. Endoscopy. 2020;52:268–75. 

 
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